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Apple Pomace Supplementation Favorably Alters Hepatic Lipid Metabolism in Young Female Sprague‐Dawley Rats fed a Western Diet
Author(s) -
Skinner Roy Christopher,
Warren Derek C.,
Lateef Soofia N.,
Benedito Vagner A.,
Bryner Randall W.,
Tou Janet C.
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.608.1
Subject(s) - pomace , steatosis , fatty liver , lipogenesis , lipid metabolism , medicine , endocrinology , fatty acid synthase , biology , chemistry , food science , disease
Apple pomace is a waste byproduct of apple processing that is high in nutrients including polyphenols, pectin, insoluble fibers, and fructose that may contribute to alleviation of some metabolic diseases. Non‐alcoholic fatty liver disease is the most common chronic liver disease in Western countries with the cause attributed to a high fat, high sucrose (Western diet) consumption. The objective of this study was to determine the effects of apple pomace supplementation on hepatic lipid metabolism. Growing (age 22–29 days) female Sprague‐Dawley rats were randomly assigned (n=8 rats/group) to consume a standard purified rodent diet (AIN‐93G), AIN‐93G +10% kcal apple pomace, Western diet (45% fat, 33% sucrose), or Western + 10% kcal apple pomace diets for 8 weeks. Histological evaluation showed rats consuming Western diets had the highest hepatic steatosis although apple pomace supplementation diet led to a lower degree of fat infiltration. Gene expression of hepatic lipogenesis by RT‐qPCR showed no significant differences of transcription factors, carbohydrate response element binding protein (ChREBP) and sterol element binding protein‐1c (SREBP‐1c), or the enzymes, fatty acid synthase (FAS) and stearoyl‐CoA desasturase‐1 (SCD‐1) among diet treatments. Rats fed Western diet had higher (p<0.01) gene expression of diacylglycerol O‐acyltransferase 2 (DGAT2) than Western + 10% apple pomace. The results suggest lower triacylglycerol synthesis reduced hepatic fat infiltration in rats fed Western diet + 10% apple pomace. In a hypercaloric state, apple pomace supplementation, attenuated hepatic steatosis induced by the consumption of a Western diet, despite the high levels of fructose in this supplement. Support or Funding Information This project was supported by Dean Daniel J. Robison, West Virginia University Davis College of Agriculture, Natural Resources, and Design and the West Virginia University Office of the Provost Fellowship. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .