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Effect of Oleanolic Acid on Inflammatory Cytokines on Rats Fed with High Fructose Diet & Metformin.
Author(s) -
Matumba Mashudu Given,
Nyakunda Trevor,
Mukwevho Emmanuel
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.603.2
Subject(s) - metformin , insulin resistance , proinflammatory cytokine , cytokine , oleanolic acid , endocrinology , tumor necrosis factor alpha , immune system , insulin , medicine , inflammation , pharmacology , chemistry , immunology , alternative medicine , pathology
Inflammatory cytokines are signaling molecules that play a crucial role in regulation of human immune response. Current research has demonstrated that inflammatory processes also play a significant role in the etiology of type‐2 diabetes (T2D) and obesity. High levels of circulating inflammatory concentration of cytokines have been linked to insulin resistance, the hallmark of T2D. In this study we have assessed the effect of oleanolic acid on some inflammatory cytokine biomarkers (TNF‐α, IL‐6, IL‐10, MCP‐1 and VEGF). Oleanolic acid is a plant derived compound that has shown some promising therapeutic effects on insulin resistance and T2D. In this study, Sprague Dawley rats were fed with high fructose diet (HFD) and also metformin. Analysis of inflammatory cytokines concentration in blood plasma was done using Bio‐Plex Pro magnetic bead‐based assay. Results showed that all five cytokines were decreased when OA was used and also increased when HFD was used. Similar pattern when gene expression was studied was also observed. These results clearly indicate that OA influences their concentration and also their gene expression. On the contrary HFD, showed the opposite by increasing both the concentration as well as the gene expression of these cytokines. In conclusion these study further suggests that OA plays a key role on inflammatory cytokine regulation and it can be used as an effective therapeutic agent to ameliorate insulin resistance and T2D. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .