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Copeptin Responses to Isotonic and Hypertonic Saline Infusion in Healthy Adults
Author(s) -
Suh HyunGyu,
Jansen Lisa T.,
Sprong Cameron,
Adams J.D.,
Butts Cory L.,
Seal Adam D.,
Scott Dylan,
Melander Olle,
Lemetais Guillaume,
Dolci Alberto,
Vanhaecke Tiphaine,
Perrier Erica T.,
Kirkland Tracie,
Kavouras Stavros A.
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.597.2
Subject(s) - copeptin , hypertonic saline , endocrinology , medicine , hematocrit , vasopressin , tonicity , chemistry , saline , plasma osmolality
Copeptin, a c‐terminal segment of arginine vasopressin (AVP) prohormone, has equimolar secretion to AVP in response to osmotic, stress‐related, and hemodynamic stimulation. For this reason, and because of the difficulties in measuring AVP directly linked to its short half‐life, copeptin has been used as a stable and sensitive surrogate marker for AVP for various clinical indications. However, the plasma copeptin response to osmotic stimulation remains poorly documented. PURPOSE Examine the plasma copeptin response to plasma hyperosmolality induced by hypertonic saline infusion. METHODS Sixty healthy adults (50% male, age: 30±1 y, weight: 78.2±15.2 kg, body mass index: 26.9±4.0 kg·m −2 ) were infused intravenously with 3% (HYPER) or 0.9% (ISO) NaCl for 2 h (0.1 ml·kg −1 ·min −1 ) in a counterbalanced, cross‐over design. Blood samples were collected every 30 minutes. Plasma osmolality (P Osm ), copeptin (P Cop ; BRAHAMS copeptin proAVP, Thermo Fisher Scientific, Germany), and sodium (P Na ) were measured, and changes in plasma volume (PV) were calculated from hematocrit and hemoglobin using the Dill & Costill equation. RESULTS Following the HYPER trial, P Na increased from 136±2 mmol ·L −1 to 146.5±3 mmol ·l −1 ( P < 0.001), with PV expansion of 18.7±7.3% ( P < 0.001). Both P Osm and P Cop increased significantly (0 min: 286±3 mmol·kg −1 and 4.5±3.7 pmol·L −1 ; 120 min: 305±4 mmol·kg −1 and 20.4±12.8 pmol·L −1 , respectively, P < 0.001). Mean (95% CI) P Cop increased with increasing P Osm : 3.2 (2.5–3.8) pmol·L −1 for P Osm ≤ 284.9 mmol·kg −1 ; 5.4 (3.8–7.0) pmol·L −1 for P Osm 285–289.9; 6.2 (4.3–8.0) pmol·L −1 for P Osm 290–294.9; 8.5 (7.1–9.9) pmol·L −1 for P Osm 295–299.9; 12.3 (10.2–14.4) pmol·L −1 for P Osm 300–304.9; and 19.2 (16.3–22.2) pmol·L −1 for P Osm ≥ 305 mmol·kg −1 . In contrast, during the ISO trial P Na remained unchanged during the 2‐h infusion (135.9±1.9 vs. 137.6±1.6 mmol·L −1 ), while PV expanded by 10.5±4.4% ( P < 0.001 compared to baseline; P < 0.001 compared to HYPER). Neither P Osm (0 min: 285±4,120 min: 287±3 mmol·kg −1 ) nor P Cop (0 min: 4.1±1.8, 120 min: 3.8±3.0 pmol·L −1 ) were affected during the ISO trial. CONCLUSION Plasma copeptin was stimulated by increased plasma osmolality during a hypertonic infusion. Support or Funding Information This research was supported by Danone Research This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .