Premium
Angiogenesis is Not Impaired in Cultured Rat Mesenteric Microvascular Networks
Author(s) -
Hodges Nicholas A.,
Murfee Walter
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.578.8
Subject(s) - angiogenesis , sprouting , basic fibroblast growth factor , stimulation , neovascularization , fibroblast growth factor , medicine , fetal bovine serum , andrology , endocrinology , biology , chemistry , pathology , growth factor , in vitro , biochemistry , receptor , botany
Angiogenesis is commonly assumed to be impaired in aged tissues. However, this preconception is challenged by evidence of increased angiogenesis during cancer and other pathological scenarios prevalent in aged models. In addition, the cumulative evidence from the literature remains non‐definitive. Hence, fundamental questions remain regarding the mechanisms by which angiogenesis is affected in aged tissues. The objective of this study was to compare the angiogenic responses in aged versus adult rat mesenteric cultured tissues. Adult (9 month) and Aged (24 month) male Wistar rat mesenteric tissue were harvested and cultured for 3 days per media group: 1) minimum essential media (MEM), 2) MEM with 10% fetal bovine serum (FBS), and 3) MEM with basic fibroblast growth factor (bFGF) (minimum of n = 8 tissues from 4 rats per group). After culture tissue microvessels were identified by PECAM labeling. FBS and bFGF stimulation induced an increase in the number of capillary sprouts per vascular area in the Adult (MEM = 2.14 ± 0.374 sprouts/mm2; FBS = 4.44 ± 0.595 sprouts/mm2; bFGF = 4.80 ± 0.447 sprouts/mm2; p < 0.05) and Aged (MEM = 3.12 ± 0.520 sprouts/mm2; FBS = 5.48 ± 0.609 sprouts/mm2; bFGF 5.98 ± 0.563 sprouts/mm2; p < 0.05) tissues compared to age group matched MEM only tissues. Capillary sprouting was not significantly different between the FBS stimulated aged and adult tissues (p = 0.216). Similarly, sprouting in the bFGF stimulated aged and adult tissues were not significantly different (p = 0.125). These results suggest that angiogenesis in aged tissues can be stimulated comparable to adult levels and that angiogenesis is not necessarily impaired. Support or Funding Information Funded by NIH R01AG049821. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .