Premium
Non‐invasive assessment of arterial stiffness correlates with age‐related vessel structure: a new Popmètre Device for Rats
Author(s) -
Amaral Sandra Lia,
Fabricio Mayara F.,
Vidal Mateus R.,
Ruiz Thalles F.R.,
Zago Anderson S.,
Vicentini Carlos A.,
Jordão Maria T.,
Michelini Lisete C.
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.578.3
Subject(s) - pulse wave velocity , arterial stiffness , medicine , lumen (anatomy) , aorta , cardiology , arterial wall , blood pressure , anatomy
Arterial stiffness measurement, evaluated by pulse wave velocity (PWV), is a predictor of cardiovascular events. Among the main factors that affect arterial stiffness are cross‐sectional area, medial wall thickening and wall‐to‐lumen ratio. Recently, a device using finger‐toe pulse wave velocity was validated for humans study, but to better understand the mechanisms that change arterial stiffness, it is necessary to use the arterial vessel for genic, proteic and histological analysis. Therefore, a new device – popmètre® – was designed for measurements of PWV in animals and it was not validated yet. The aim of this study was to correlate the PWV using a new device for animal studies with vessel structure in rats of different ages. Forty‐two Wistar rats were allocated into 3 groups of different ages: Young: 1 month; Adult: 4 months and Older: 12 months. Two photodiodes were positioned on the right thoracic and pelvic members for PWV measurement, on anesthetized rats. Then, aorta, carotid, femoral and renal arteries were extracted and prepared for histological analysis (cross sectional area – CSA and wall thickness). Results of PWV and morphometric analysis were then correlated using Pearson correlation test. As expected, PWV was 46% higher in adult rats compared with young rats and 20% higher in aged rats compared with adult ones. Cross sectional area was significantly higher in aged rats compared with young rats in aorta (+151%), carotid (+94%), femoral (+62%) and renal (251%) arteries. Wall thickness was not altered among groups. A significant correlation was observed between CSA and PWV in all arteries: aorta (r=0.5642; p<0.001); carotid (r=0.6854 p<0.001); femoral (r=0.6642 p<0.001) and renal ( r=0.7817; p<0.0001). Wall thickness did not correlate with PWV. The results of this present study allow us to suggest that PWV measurement, obtained by a new device pOpmètri® for small animals, was able to detect age‐related vessel structure in rats. Support or Funding Information São Paulo Research Foundation (FAPESP) and Coordination for the Improvement of Higher Education Personnel (CAPES). This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .