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Alterations in human neutrophil function by Bisphenol A
Author(s) -
Balistrieri Angela,
Meier Angela,
Corriden Ross
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.574.9
Subject(s) - chemotaxis , innate immune system , neutrophil extracellular traps , chemistry , calcium , immune system , reactive oxygen species , extracellular , nadph oxidase , microbiology and biotechnology , inflammation , receptor , biochemistry , immunology , biology , organic chemistry
Bisphenol‐A is a synthetic organic compound frequently present in consumer plastics, including plastic‐lined cans, water bottles, toys, and teeth sutures. Previous studies have shown that BPA can produce adverse health effects that include defects in reproductive function and altered prenatal/childhood development. However, little is known regarding the effects of BPA on immune function. In this study, we assessed the effect of BPA on human neutrophils, a critical component of the innate immune system's defense against pathogens. We found that BPA induces a concentration‐dependent increase in reactive oxygen species generation by PMNs that is inhibited by the estrogen receptor β antagonist PHTPP and the NADPH oxidase inhibitor diphenyleneiodonium chloride. Furthermore, incubation with the membrane‐permeable calcium chelator BAPTA‐AM and/or removal of extracellular calcium inhibited BPA‐induced ROS production, indicating that the process is calcium dependent. Transwell chemotaxis assays revealed that BPA exposure reduces the chemotactic capacity of PMNs in a gradient of the bacterial cell wall component f‐Met‐Leu‐Phe, a potent chemoattractant. Exposure to BPA also inhibits the ability of neutrophils to kill methicillin‐resistant Staphylococcus aureus , a leading human pathogen. Our findings reveal that BPA alters the in vitro function of PMNs, including ROS production, chemotaxis and bacterial killing, and raise the possibility of altered innate immunity in vivo , especially in those with compromised immune function and who can be exposed to BPA in a wide variety of products. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .