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Effect of Calcium Sulfide Nanoclusters in Cell Proliferation of Malignant Lung and Pancreatic Cell Lines
Author(s) -
Torres Geraline Trossi,
Rosado María Figueroa,
Forti Kevin Muñoz,
Suarez Edu
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.565.5
Subject(s) - medicine , cancer research , nanoclusters , pancreatic cancer , cell , cell culture , cell growth , oncology , chemistry , pathology , cancer , biology , biochemistry , organic chemistry , genetics
Lung and pancreatic cancer are leading cause of cancer deaths in the United States. Despite significant developments in treatments, survival rate for lung and pancreatic cancer patients is low. The diagnosis of these cancers is usually done at later stages, are very aggressive and when treated it affects negatively the quality of life of the patients. Recently, there have been advances in nanomedicine particularly in drug delivery and carrier systems developed to treat many types of cancers including lung, pancreas, breast, and prostate, among others. Calcium and sulfur are expected to be biocompatible with human body due the availability of these elements in our system. We propose the use of Calcium Sulfide (CaS) nanoclusters as an anti‐proliferation agent acting selectively in malignant non‐small cell lung adenocarcinoma (NSCLC HCC827) and pancreatic epithelial carcinoma cells (Mia Paca‐2) without affecting non‐malignant lung cells (MRC5). We hypothesize that CaS nanoclusters will decrease the cell replication in HCC827 and in Mia Paca‐2 cell lines without affecting the MRC5. Cells were treated with DMSO [1%] (vehicle control), etoposide [10μM] (positive control), or CaS nanoclusters [3.8μM] (experimental treatment) at 0‐hours. We performed each assay in quadruplicates according to their incubation time: 24, 48 and 72 hours after a single‐dose exposure to the correspondent treatments. To evaluate the effects of the CaS nanoclusters, we performed BrdU Proliferation Assay to quantify the newly synthesized DNA. The preliminary results showed that there was no significant changes in the pancreatic cell lines Mia Paca‐2 and HCC827 treated with CaS when compared to the controls at 24, 48, and 72 hours. In the MRC5 cell line there was only a borderline significance of 0.056 at 48 hours, which showed an increase in proliferation that confirm previous findings using other methods in our lab. In the following step, we plan to evaluate the effect of CaS in apoptotic and autophagy markers using the same cell lines. Support or Funding Information NIH‐NIGMS #2R25GM096955 This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .