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Studies on identification of genes associated with neuronal memory using of rat hippocampal neurons
Author(s) -
Opoku Ruth,
Ahmed Ijaz,
Patwary Mohsin,
NureKamal Alam
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.551.4
Subject(s) - neurite , hippocampal formation , laminin , microbiology and biotechnology , extracellular matrix , fibronectin , neuroscience , neuron , biology , in vitro , biochemistry
We have previously demonstrated that extracellular matrix (ECM) proteins facilitate neurite extension of rat cerebellar granule neurons (CGNs) in culture. We have also identified the functional domain(s) of these proteins involved in neurite outgrowth. Hippocampal neurons play an important role of storing memory in animals. We propose a hypothesis that “hippocampal neurons can be used to identify genes associated with storage of memory in animals”. In this report we developed a ECM‐coated culture surface to growth primary rat hippocampal neurons and determined their neurite outgrowth. We have also compared the neurite outgrowth of CGN and hippocampal neurons. Cerebellar granule neurons, cortical neurons (CN), and hippocampal neurons were plated onto the cover slips coated with extracellular matrix proteins (laminin‐1, fibronectin, and collagen) at a density of 105 neurons per ml. The neurons were allowed to extend neurites for 24 hrs in NBM/B27/KCl. The extent of neurite outgrowth was then determined using carboxyfluorescein diacetate (CFDA) labeling. Images of the cultures were captured and analyzed with the NIH Image software. A sample of 100 neurons with well‐defined processes were studied for each condition. Laminin and fibronectin‐coated surfaces induced neurite outgrowth of Cerebellar granule neurons, cortical neurons, and hippocampal neurons. It was found that collagen stimulates neuirte outgrowth of CGN, while collagen inhibited neurite outgrowth of hippocampal neuron. We have developed a condition for culture of hippocampal neurons. By immunostaining, immunoblot, and RT‐PCR analysis we found alteration of expression of some genes genes involved in neurite outgrowth, Results of these studies with be presented in this report. These hippocampal neurons will be used to apply different types of stimuli and identify genes involved in saving memory in neurons. Identification of genes associated with saving memory might be useful in developing a therapy for neurological disorders including Alzheimer's, Parkinson's diseases. Support or Funding Information NIH‐RISE This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .