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High fat diet risk factor for gingival Immunosuppression and cancer
Author(s) -
Kaur Kawaljit,
Eibl Guido Anahid,
Jewett Anahid
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.547.9
Subject(s) - cytotoxicity , adipose tissue , spleen , immune system , kras , endocrinology , chemistry , medicine , biology , immunology , cancer , in vitro , biochemistry , colorectal cancer
Objective High fat diet leading to obesity has been associated with many diseases including cancer. We determined the effect of high fat diet and obesity in the phenotype and function of immune cell infiltrates within different tissues including gingival tissues of mice with wild type and targeted KRAS mutations in the pancreas. Experimental methods Conditional LSL‐KRAS G12D and p48‐Cre or PDX‐1‐Cre mice were bred and their offsprings were used for the studies. Mice with both the Kras G12D and Cre allele were designated as mutant mice (KC). Mice with neither the Kras G12D nor the Cre allele were used as wildtype (WT). Mice were fed either a high fat, high calorie diet (HFCD) or a control diet (CD) for 4 to 6 months before they were used for experiments. NK cytotoxicity was determined by 51Cr release assay and cytokine secretion by ELISA. Results The Following profiles were obtained for NK cell cytotoxicity and secretion of IFN‐g from NK cells purified from spleen of KC or WT mice fed either with CD or HFCD (WT(CD)>WT(HFCD)>KC(CD)>KC(HFCD). Immune cells from blood and pancreas exhibited similar pattern of cytotoxicity to those obtained from the spleen but inverse profile of IFN‐g and IL‐6 secretion (WT(CD)