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GlycoSense™: A Rapid Method for Monitoring In Vitro Glycoengineering
Author(s) -
Yang Loretta,
Saunders Matthew J.,
Samli Kausar N.,
Woods Robert J.
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.544.20
Subject(s) - glycan , glycosylation , glycoprotein , multiplex , in vitro , flow cytometry , chemistry , computational biology , biochemistry , biology , microbiology and biotechnology , bioinformatics
The use of enzymes to modify glycans, in order to generate uniform protein glycosylation for optimal bioactivity, or to test glycan‐structure function hypotheses, or in chemo‐enzymatic synthesis, has emerged as a transformative technology. However, there are no convenient cost‐effective methods for monitoring the progress of the enzyme reactions during in vitro glycoengineering. Lectenz ® Bio has developed an innovative technology called GlycoSense™ that will permit in vitro glycoenzyme reactions to be monitored in near real‐time using flow cytometry and multiplex microspheres (beads). The GlycoSense™ method detects binding between glycans and glycan‐specific reagents that are conjugated to spectrally‐unique beads. By combining the individual bead‐based reagents into a multiplexed array, a profile of key glycan features can be obtained in less than a minute on a basic cytometer. We illustrate the performance of the GlycoSense™ method using glycans and glycoprotein standards, and demonstrate its utility by monitoring changes in glycosylation patterns upon treatment with glycoenzymes. The GlycoSense™ approach will address the unmet need for a rapid tool to monitor protein glycosylation during in vitro glycoengineering. Support or Funding Information National Institutes of Health (U01CA221213, R43GM123863) This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .