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Characterization of Acid Phosphatase Adsorption to Montmorillonite
Author(s) -
McTaggart William Stuart
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.533.75
Subject(s) - montmorillonite , phosphorus , adsorption , chemistry , phosphatase , acid phosphatase , cycling , enzyme , biochemistry , environmental chemistry , organic chemistry , archaeology , history
Phosphorus is important in agriculture because it limits the growth of plants and can influence the size of crop yields. The phosphorus cycle takes significant time in replenishing phosphorus lost to runoff and human activities — in the short‐term, phosphorus is non‐renewable. This project aims to better understand how naturally‐occurring enzymes influence the phosphorus cycle, particularly how these enzymes adsorb to soil. The project is taking advantage of microplate assays to analyze how acid phosphatase (AP) from wheat interacts with montmorillonite, a clay. Key experiments investigate the time it takes for the binding of AP to montmorillonite to equilibrate, the effect of AP concentration on adsorption and the total amount of binding. The data showed that binding came to equilibrium in approximately two hours, that greater binding occurs with more AP, and that the percentage of AP adsorbed increases significantly with the amount of montmorillonite. A fuller understanding of phosphorus cycling may allow for manipulation of the cycle to make phosphorus use more efficient. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .