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Using embryonic zebrafish to evaluate the effects of exposure to novel bisphenol analogues
Author(s) -
Bruton Laura N.,
Zhou Xinrui,
Timmons Shan C.,
Morrissette Jeffrey M.
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.533.63
Subject(s) - zebrafish , bisphenol a , bisphenol , endocrine disruptor , toxicity , endocrine system , human fertilization , fish <actinopterygii> , biology , chemistry , pharmacology , endocrinology , medicine , biochemistry , anatomy , hormone , fishery , gene , organic chemistry , epoxy
Bisphenol A (BPA) is a well‐established endocrine disruptor that has been shown to induce developmental and behavioral changes in both human and animal models. Gestational exposure of embryonic zebrafish to low nanomolar doses of bisphenol A (or S) altered hypothalamic neurogenesis during development and induced hyperactive swimming behaviors. There are many potential bisphenol analogues, but little is known about their toxicity or ability to act as endocrine disruptors. We have synthesized a small library of novel bisphenol analogues and are testing their toxicity in a zebrafish larval toxicity assay. We are also using an ELISA‐based estrogen binding assay to assess the analogues' affinity for estrogen receptors. We will be exposing zebrafish embryos to ranging concentrations of bisphenol analogues during time periods of 10–16 hpf (hours post‐fertilization), 16–24 hpf, and 24–36 hpf. At 5 dpf (days post‐fertilization) zebrafish locomotor activity in control and analogue exposed fish will be assessed using video‐tracking software quantifying bursts of hyperactivity. Results from these experiments will be discussed. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .