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GPR56/ADGRG1 activation induces IL‐6 production in melanoma cell via the Gα 12/13 ‐ RhoA‐ROCK pathway
Author(s) -
Huang KuanYeh,
Chiang NienYi,
Lin HsiHsien
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.533.35
Subject(s) - rhoa , melanoma , cancer research , receptor , cell , chemistry , signal transduction , biology , microbiology and biotechnology , biochemistry
GPR56/ADGRG1, a member of the adhesion G protein‐coupled receptor family, is important for tumor progression. The role of GPR56 in melanoma development is unresolved. Here, we showed that GPR56 receptor ligation by immobilized CG4 mAb in melanoma cell enhanced the production of IL‐6, which promoted melanoma cell migration and invasion. siRNA‐mediated Gα 12/13 knockdown significantly reduced CG4‐induced IL‐6 production in melanoma cell. Likewise, the IL‐6 level was decreased in melanoma cells expressing the dominant negative RhoA N19, treated with exoenzyme C3, or the ROCK inhibitors (Y27632 and H1152). We conclude that GPR56 activation‐induced IL‐6 production is mediated through the Gα 12/13 ‐RhoA‐ROCK signaling pathway. IL‐6 in turn promotes melanoma cell migration and invasion. Our results indicate a critical role of GPR56 in melanoma progression. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .