Premium
High‐ throughput Carbonic Anhydrase Activity and Inhibitor Screening Assays
Author(s) -
Hazra Suratna,
Tchaga Grigoriy,
Yan Gordon July
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.530.12
Subject(s) - carbonic anhydrase , chemistry , carbonic anhydrase ii , bicarbonate , enzyme , biochemistry , carbon dioxide , organic chemistry
Carbonic Anhydrase (CA) is a zinc based metalloprotease present in at least 14 different isoforms in mammals. It catalyzes the reversible hydration of carbon dioxide to form bicarbonate and proton, thus playing an important role in pH and CO 2 homeostasis. Changes in CA activity are associated with various diseases such as glaucoma, type II diabetes mellitus, lung and liver diseases etc. As CA is also an important target for cancer therapy developing high‐throughput carbonic anhydrase activity and inhibitor screening assays is very important. Carbonic anhydrase activity or carbon dioxide hydration rate is widely measured by using potentiometer or a stopped‐flow instrument as reflected by the change in pH over time. However, this method is not suitable for high‐throughput assay. Consequently, in BioVision, Inc. we have launched the first high throughput Carbonic anhydrase activity assay kit, detecting the esterase activity of this enzyme. This kit utilizes the zinc‐hydroxide mechanism for carbon dioxide hydration and hydrolysis of an ester substrate resulting in a release of a chromophore signal that is proportional to the enzyme activity. Support or Funding Information Biovision Inc. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .