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Characterization of the Dual Pathways for B. halodurans Asparaginyl‐tRNA Formation
Author(s) -
Schroeder Caitlin M.,
Sheppard Kelly
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.526.35
Subject(s) - transfer rna , asparagine , amino acid , biochemistry , computational biology , biology , translation (biology) , rna , chemistry , messenger rna , gene
Protein synthesis requires the attachment of an amino acid to its cognate transfer RNA (tRNA). Two distinct pathways for attaching asparagine (Asn) to tRNA Asn are known: the direct pathway, in which Asn is directly attached to tRNA Asn by the enzyme AsnRS; and the indirect pathway, in which Asn is attached to tRNA by a non‐discriminating AspRS and GatCAB. Bacillus halodurans uses both routes for Asn‐tRNA Asn formation. To better understand why B. halodurans possesses both pathways, we have purified its AsnRS and AspRS in order to test and compare their activities under different physiologically relevant conditions such as in the presence of reactive oxygen species and changes in pH. This research will provide insight into how B. halodurans has adapted to survive in different environmental conditions. Support or Funding Information National Science Foundation (MCB‐1615770) This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .