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Evidence for direct interaction between RNA polymerase and the small ribosomal subunit.
Author(s) -
Blaha Gregor,
Diggs Stephen,
Conn Adam B.,
Williams Preston,
Wang Yinsheng
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.526.24
Subject(s) - rna polymerase i , ribosome , ribosomal rna , rna polymerase , polymerase , eukaryotic ribosome , rna polymerase ii , transcription (linguistics) , eukaryotic large ribosomal subunit , biology , microbiology and biotechnology , ribosomal protein , 5.8s ribosomal rna , protein subunit , rna polymerase iii , rna , genetics , gene , promoter , gene expression , linguistics , philosophy
In E. coli , RNA polymerase can directly bind ribosomes. This direct interaction may enable the coordination of the RNA polymerase and ribosome activities during transcription‐translation coupling. The coupling of both processes is exploited for gene regulation to enable immediate response to environmental changes. To establish transcription‐translation coupling, small ribosomal subunits have to be recruited to the nascent RNAs to initiate translation. Here, we report the recruitment of the small ribosomal subunit by RNA polymerase. Similar to the binding of ribosomes to RNA polymerase, the small ribosomal subunit binding to RNA polymerase is modulated by the conformation and functional state of both macromolecules. The RNA polymerase binding site on the small ribosomal subunit was identified by chemical crosslinking and is centered on the mRNA exit site of the small ribosomal subunit. Support or Funding Information University of California, Riverside (start‐up funding) This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .