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Long‐term dietary nitrite supplementation is effective in reducing cardiovascular disease risk factors in older, obese rats
Author(s) -
Kadel Jacob L.,
Van Schaeffer G,
Bates Melissa L.,
Sindler Amy L.
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.517.4
Subject(s) - medicine , blood pressure , nitrite , endocrinology , obesity , sodium nitrite , endothelial dysfunction , chemistry , food science , nitrate , organic chemistry
Cardiovascular diseases (CVD) persist as the leading cause of death. CVD risk increases with advancing age, and is further exacerbated in settings of obesity. Sodium nitrite supplementation improves cardiovascular abnormalities in several models of dysfunction. Given the association between aging, obesity and CVD risk, we tested the hypothesis that long‐term sodium nitrite supplementation would reduce CVD risk factors in older, obese rats. At 6 months of age, male Fisher 344 rats were randomly split into two groups; animals fed HFD (60%(Research Diets); n = 10) with or without nitrite‐supplemented water (50 mg/L; HFD‐NO 2 − ; n = 10) for 10 months. Age‐matched rats fed regular rodent chow (2920X (Teklad); n = 10) served as controls (CON). As expected, rats on a HFD for 10 months were heavier vs. CON (598 ± 11 vs. 432 ±10 gms; p <0.0001), which nitrite attenuated (533 ± 14 gms; p = 0.0021). DEXA scans revealed that the reduced body weight in the HFD‐NO 2 − rats was accompanied with reduced body fat % (28 ± 0.6 vs. HFD 31 ± 0.4%; p = 0.0046), while having no effect on lean % (HFD‐NO 2 − : 48.1 ± 0.4 vs. HFD: 47.5 ± 0.2%; p = 0.30). Compared to CON, HFD rats had increased systolic blood pressure (SBP: 120 ± 3.81 vs. 136 ± 5.40 mmHg; p = 0.03), diastolic blood pressure (DBP: 75 ± 2.49 vs. 87 ± 4.24 mmHg; p = 0.04) and mean arterial pressure (MAP: 90 ± 2.71 vs. 103 ± 4.53 mmHg; p =0.03), which was completely prevented in HFD‐NO 2 − (SBP: 114 ± 4.07 mmHg; p = 0.004, DBP: 74 ± 4.15 mmHg; p = 0.04, and MAP: 87 ± 4.05 mmHg; p = 0.016, all vs. HFD). Furthermore, HFD‐NO 2 − rats had reduced fasting glucose when compared to HFD rats (97 vs. 1.3 vs. 104 ± 2.6 mg/dL; p = 0.02). Taken together, these data demonstrate that nitrite supplementation may have therapeutic potential for treating and/or preventing CVD risk factors that are associated with aging and obesity. Support or Funding Information Supported by NIH K01AG047626, University of Iowa College of Liberal Arts and Sciences Old Gold Summer Fellowship, and University of Iowa Department of Health and Human Physiology This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .