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Protective effect of silybin against verapamil‐induced hepatotoxicity in rats
Author(s) -
Hassan Sherif,
Rizk Ayman,
Motawie Ahmed G.,
Abdelfattah Shereen M.,
Ramaraj Pandurangan
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.511.3
Subject(s) - alkaline phosphatase , pharmacology , chemistry , medicine , liver injury , endocrinology , enzyme , biochemistry
Background Verapamil (VPA) is considered one of the anticonvulsant and mood‐stabilizing drug used for treating seizures and epilepsy. Several mechanisms have been suggested for VPA hepatotoxicity most of them are associated with oxidative stress. Silybin (silymarin) is a flavonoid known for its anti‐inflammatory, antioxidant properties. It may be possible to use silybin to protect against VPA‐induced hepatotoxicity. Aim of study The aim was to study the protective effect of silybin against VPA‐induced hepatotoxicity in rats. Methods 40 rats were equally divided into 4 equal groups of 10 rats: Group I: (Control), Group II (sham), Group III (VPA), and Group IV (silybin+VPA). Liver sections were subjected to histological and immunohistochemical study. Blood samples were taken to test for liver enzymes. Histomorphometric and statistical analysis were performed. Results Liver sections of Group III showed focal areas of degenerated hepatocytes and dilated congested central veins. The hepatocytes revealed small vacuoles, pyknotic nuclei and marked inflammatory cellular infiltrations. There were dilation and congestion of the hepatic sinusoids. The hepatocytes revealed weak PAS reaction and strong Caspase 3 immunostaining. There were marked increase in the liver enzymes including alkaline phosphatase (ALP), aspartate amino transferase (AST) and alanine amino transferase (ALT). Group IV showed remarkable improvement and preservation of the hepatic architecture apart from mild pathological changes. Most hepatocytes were apparently normal. Strong positive PAS reaction and scanty weak Caspase 3 immunostaining were noticed. The liver enzymes were within normal as compared with the control group. The data were analyzed using SPSS and were described as mean ± standard deviation (SD). Then statistical comparison was performed using one‐way analysis of variance. Differences between mean values were assessed using a t test. A P<.05 was considered statistically significant. Conclusion Silybin could protect against verapamil‐induced hepatotoxicity in rats. Support or Funding Information This study is self‐funded This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .