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Insulin and ketones elicit disparate effects on mitochondrial uncoupling in adipose tissue
Author(s) -
Bikman Benjamin T.
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.41.2
Subject(s) - adipose tissue , insulin , thermogenin , endocrinology , uncoupling protein , brown adipose tissue , medicine , mitochondrion , biology , thermogenesis , wasting , chemistry , microbiology and biotechnology
Objective Insulin is a powerful regulator of metabolic function, promoting a general storage of nutrients. In contrast, ketones, inversely regulated by insulin, lead to enhanced energy use and wasting. The purpose of this study was to explore the effects of insulin and ketones on mitochondrial coupling status in adipose tissue. Methods We used cell, rodent, and human models, to varying degrees. 3T3‐L1 adipocytes were treated at various times with insulin or ketones. Similarly, rodents were chronically injected with insulin or placed on ketogenic diets. Finally, human fat biopsies were obtained from in states of ketosis. In all models, mitochondrial respiration was determined and, where available, uncoupling protein (UCP) 1 was measured. Results In cells and rodents, insulin reduced mitochondrial respiration and, in rodents, reduced indirect calorimetry. In all models, including human adipose, ketones increased respiration rates and UCP1 levels. Conclusions Insulin and ketones elicit disparate effects on mitochondrial uncoupling in adipose tissue. Whereas insulin reduces uncoupling, resulting in more tightly coupled electron transport, ketones enhanced uncoupling, resulting in energy wasting. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .