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The influence of high fiber diet and probiotic supplementation in a murine model of colitis
Author(s) -
Owens Josh,
Saeedi Bejan,
Darby Trevor,
Jones Rheinallt
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.406.2
Subject(s) - lactobacillus rhamnosus , probiotic , colitis , immune system , lamina propria , cytoprotection , inflammatory bowel disease , immunology , biology , medicine , food science , disease , pathology , oxidative stress , bacteria , epithelium , genetics
Probiotics are therapeutic agents administered to patients with IBD. In addition, it has recently been hypothesized that a high fiber diet may synergize with probiotics to exert an elevated cytoprotective effect on host tissue during colitis. Thus, we investigated the extent to which both orally administered probiotics and a high fiber diet protected intestinal tissue in a murine model of colitis. We administered the well‐studied probiotic, Lactobacillus rhamnosus GG (LGG) to 8 week‐old mice for two weeks before a 7‐day challenge with 3% Dextran Sodium Sulfate (DSS). Protection was assessed in terms of disease activity index and histology. Mice given LGG or a high fiber diet conferred modest protection from DSS‐induced colitis compared to controls. However, the cytoprotective effect was significantly strengthened in mice fed a high fiber diet in conjunction with LGG. Flow Cytometry analysis of immune cells in the colonic lamina propria revealed an immune‐modulatory effect of LGG, which was elevated under a high fiber diet. Together, these data suggest that a high fiber diet plus a probiotic can lead to enhanced cytoprotection against DSS‐induced colitis compared to either LGG feeding, or a high fiber diet alone. Support or Funding Information Research supported by NIH grant R01DK98391 This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .