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New technologies to interrogate G protein‐coupled receptor signaling
Author(s) -
Kruse Andrew
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.382.4
Subject(s) - g protein coupled receptor , receptor , signal transduction , microbiology and biotechnology , biology , agonist , computational biology , biochemistry
G protein‐coupled receptors (GPCRs) are the largest family of cell‐surface receptors in humans, and they play critical roles in processes ranging from neurotransmission to the maintenance of metabolic homeostasis. In order to better understand the molecular basis for GPCR responses to endogenous activators and synthetic drugs, we have developed a new method to track GPCR signaling in live cells using time‐resolved proximity labeling (GPCR‐APEX). This method reveals the collection of proteins a GPCR encounters following activation, and enables quantitative measurement of receptor fate following agonist stimulation. In addition to tracking GPCR signaling, we have developed new methods for the discovery of synthetic antibody fragments as modulators of receptor conformational change and signal transduction. Together with GPCR‐APEX, synthetic antibody fragment modulators allow investigation and control of GPCR signaling in new ways. Support or Funding Information This work was supported by grants from the Smith Family Foundation, The Vallee Foundation, and an NIH Early Independence Award (5DP5OD021345). This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .