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Initiation, targeting and sculpting of the phagophore
Author(s) -
Hurley James H.
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.379.2
Subject(s) - autophagosome , autophagy , microbiology and biotechnology , cytosol , phosphatidylinositol , organelle , kinase , biology , programmed cell death , chemistry , biochemistry , apoptosis , enzyme
Autophagy is a conserved mechanism that is essential for cell survival in starvation and for cellular homeostasis. The autophagosome and the proteasome comprise the two main pathways for the degradation of proteins. Autophagy proceeds by the engulfment of bulk cytosol and organelles by a cup‐shaped double membrane sheet known as the phagophore, which matures into the autophagosome. Two protein complexes control the initiation of the phagophore: the Atg1/ULK1 kinase complex and the class III phosphatidylinositol 3‐kinase complex I (PI3KC3‐C1). Our laboratory has been applying crystallography, electron microscopy, mass spectrometry, and allied biophysical techniques, in conjunction with cell biology approaches, to understand how these complexes are organized in three dimensional space and time, and how their architectures underpin their regulation and activity. Support or Funding Information NIGMS R01 111730 and P01 051487 This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .