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Inhibition of FPR2 Impaired Leukocyte Get‐in Signal and Triggers Non‐Resolving Inflammation in Heart Failure
Author(s) -
Kain Vasundhara,
Wright Griffin M.,
Halade Ganesh V.
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.287.4
Subject(s) - inflammation , medicine , integrin alpha m , spleen , proinflammatory cytokine , formyl peptide receptor , myocardial infarction , heart failure , pharmacology , infiltration (hvac) , lipopolysaccharide , immune system , immunology , receptor , chemotaxis , physics , thermodynamics
Non‐resolving inflammation is a prominent trigger for heart failure (HF)‐related morbidity and mortality following myocardial infarction (MI). Post‐MI activated leukocyte expressed formyl peptide receptor 2 (FPR2) belongs to the essential for inflammation‐resolution mechanisms. However, the role of FPR2 in leukocyte kinetics in acute HF is incompletely understood. Here, we aimed to determine whether pharmacological inhibition of FPR2 would impair leukocyte trafficking to the site of cardiac injury using WRW4 (FPR2 antagonist). For this, male C57BL/6 (8 to 12 week) mice were subjected to acute HF using permanent coronary artery ligation. FPR2 inhibitor WRW4 (1μg/kg/day; subcutaneous) was injected 3 hours post‐MI and saline‐injected‐mice served as MI‐control. Inhibition of FPR2 impaired leukocyte trafficking (“get‐in” signal) that altered post‐MI immune responsive clearance in the infarcted LV and spleen. Leukocyte kinetics, measured using flow cytometry, showed there was an overall decrease in CD45 + /CD11b + ; 23.3±2% in WRW4‐injected mice when compared with MI‐control (49.1±2%) in the LV. FPR2 inhibitor, WRW4, decreased FPR2 in the LV and spleen tissue, and mice displayed increase F4/80 + /Ly6C hi macrophages (14.8±2%) compared to the MI‐control group (10±1%) in LV, this is indicative of amplified proinflammatory profile. Inhibition of WRW4 primes immature neutrophil infiltration Ly6G int and intensifies the Ccl2 (all p<0.05) expression compared to MI‐control in the infarcted LV post‐MI. WRW4 increased transcripts of pro‐inflammatory markers TNF‐α and IL‐1β, with a decrease in expression of Arg‐1 ( p<0.05) in the infarcted LV compared to MI‐controls is suggestive of non‐resolving or immune suppressive inflammation. In summary, FPR2 inhibition using WRW4 altered leukocyte get‐in signal leading to the onset of frustrated resolution signaling in acute HF post‐MI. Support or Funding Information HL132989 to GVH and 16POST318 to VK This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .