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Optical Imaging Tools for Elucidating the Roles of Anions in Cellular Signaling
Author(s) -
Dodani Sheel
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.123.1
Subject(s) - context (archaeology) , signal transduction , chemistry , intracellular , chemical biology , microbiology and biotechnology , neuroscience , computational biology , biology , biophysics , nanotechnology , biochemistry , materials science , paleontology
Living systems have evolved finely tuned mechanisms to coordinate transporters, chaperones, and non‐proteinaceous ligands to spatially and temporally position ions for normal physiology. In this context, freely diffusing and protein‐bound anions link to signal transduction, regulation of intracellular pH, and even detoxification. However, a breakdown in cellular homeostasis can lead to anion dysregulation in a wide range of diseases including neuropathic pain, cancer, and cystic fibrosis suggesting that anions could be signals of cellular status. Despite this biological significance, chemical biology approaches to identify the cellular stores, protein targets, and signaling roles of biologically relevant anions, are still underdeveloped, but could provide new insights for the role of anions in human health and disease. To this end, in this presentation I will describe the development and applications of turn‐on fluorescent biosensors for the real‐time imaging of anions in biological systems. Support or Funding Information Acknowledgment is made to the Welch Foundation (Grant No. AT‐1918‐20170325). This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .