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Effect of marked weight loss on adiponectin plasma concentration and adipose tissue expression in extremely obese subjects
Author(s) -
Coughlin Carrie Christine,
McCrea Jennifer,
Eagon J. Christopher,
Halpin Valerie,
Finck Brian,
Klein Samuel
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.lb96-c
Subject(s) - adiponectin , adipose tissue , medicine , endocrinology , weight loss , obesity , chemistry , insulin resistance
Adiponectin is the major secretory protein produced by adipose tissue and may be an important regulator of insulin action. Although weight loss improves insulin sensitivity, its effect on plasma adiponectin concentration and adipose tissue expression is unclear because of conflicting results from different studies. The ambiguity could be due to differences among studies in subjects’ percent weight loss. Therefore, this study's aim was to evaluate the effect of marked weight loss on adiponectin plasma concentration and adipose tissue expression. Adiponectin plasma concentration and subcutaneous abdominal adipose tissue (SAAT) expression was determined, using ELISA and quantitative RT‐PCR, in 7 obese subjects (BMI=58.7±4.3 kg/m 2 ) before and 1 year after gastric bypass surgery. One year after surgery, subjects lost 33±5% of their initial body weight, and BMI decreased to 39.7±4.7 kg/m 2 (p<0.001). Adiponectin plasma concentration increased by 66±28%, from 4.8±0.7 to 7.6±1.2 μg/mL (p=0.017) and SAAT expression increased 29±12 fold, from 21±14 to 598±70 arbitrary units (p=0.018). These data demonstrate that marked weight loss in obese subjects causes a simultaneous increase in SAAT adiponectin gene expression and plasma concentration. Therefore, weight loss upregulates SAAT adiponectin production, which likely is responsible for the increase in plasma concentrations. Supported by NIH grant DK37948.