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Effects of conjugated linoleic acid (CLA) on metabolic hormones and adipokines in geriatric mice
Author(s) -
Baker Shan H,
Sanders Sara R,
Zibadi Sherma,
Watson Ronald R,
Baumgard Lance H
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.lb83-d
Subject(s) - resistin , medicine , leptin , endocrinology , adipokine , insulin , hormone , chemistry , conjugated linoleic acid , adipose tissue , fatty acid , biology , linoleic acid , obesity , biochemistry
CLA decreases the fat content in elderly rodents, but its effect on metabolic hormones and adipocyte‐secreted cytokines remains unclear. Female geriatric (25.5 mo old) C57BL/6 mice were fed isoenergetic diets containing 0 or 1.68% methyl esters of CLA (50/50 cis ‐9, trans ‐11 and trans ‐10, cis ‐12) for 42d. A full description of CLA effects on carcass composition, organ weights, and fatty acid composition was previously presented (Baker et al., 2005). Briefly, CLA decreased carcass fat content by 51%, increased carcass protein and ash content by 49 and 38% and markedly increased liver mass (61%). Blood was collected post mortem by cardiac puncture. Serum was analyzed using a LINCOplex™ multiplex immunoassay kit (LINCO Research, St. Charles, MO) for insulin, leptin, resistin, IL‐6, MCP‐I and PAI‐1. Dietary CLA increased (P<0.05) circulating PAI‐1 (71%), and tended to increase insulin (87%; P<0.06) and IL‐6 (155%; P<0.12). CLA decreased (P<0.01) systemic leptin (86%) levels, but had no effect on either MCP‐I or resistin concentrations. Carcass fat content was correlated (P<0.05) positively with serum leptin (r=0.82) and negatively with PAI‐1 (r = 0.68). Liver fat levels tended to be positively correlated with serum insulin levels (P<0.07), though carcass fat content was not. Circulating MCP‐I and PAI‐1 levels were positively correlated (P<0.05; r=0.49) but no associations were detected between the other measured hormones/cytokines. In addition to dramatically altering body composition, CLA has marked effects on some bioenergetic hormones and inflammatory cytokines in elderly mice.