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Anthocyanin (ACN) Stability in Cell Culture Media
Author(s) -
Wu Xianli,
Prior Ronald L.,
Burt Tara D.,
Bell David R.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.lb83-c
Subject(s) - chemistry , petunidin , malvidin , anthocyanin , cyanidin , food science
Anthocyanins (ACNs) are potential oxygen radical scavengers that have coronary vasoactive and vasoprotective properties. Cell or tissue culture systems have been used to examine the bioactivity and mechanisms of action of ACNs on the vascular system. However, due to their unique chemical structure, the stability of ACNs is a major concern in studies of this nature. In this study, three ACN enhanced extracts prepared from chokeberry (CB), bilberry (BB) and Elderberry (EB) (10 mg/L) were added in Dulbecco's Modified Eagles Media (HEPES buffered and phenol red free with bicarbonate) to evaluate their stability after 1h, 2h, 4h, 8h, 16h and 24h incubation at 37°C. Identification and quantification of ACN was carried out using a reverse phase HPLC‐ESI/MS/MS system. ACNs were unstable in this media. The half life of most ACNs tested was less than 5 h. However, different ACNS exhibited differences in stability. Aglycone seemed to be the major factor determining the stability for ACNs with similar sugar patterns. Delphinidin ACNs were least stable with a half life of only 0.43±0.01 h (n=3), followed by petunidin (2.34±0.04 h, n=3); malvidin (4.04 h, n=1); penonidin (4.31 h, n=2); and cyanidin (4.45±0.60 h, n=10). A complex ACN (cyanidin 3‐sambubioside‐5‐glucoside) was more stable than cyanidin 3‐glucoside (8.76 h vs 4.05 h). Results from this study indicate that caution should be used in attributing changes in bioactivity measured in culture to the original ACNs, when degradation products could be responsible for the observed responses particularly when extended incubation times are used.