Premium
Protein profiling of matched human cell lines by Antibody Microarray 500
Author(s) -
Marinescu Corina,
Kozlowski Mark
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.lb66-b
Subject(s) - antibody microarray , protein microarray , microarray , microbiology and biotechnology , western blot , microarray analysis techniques , cell culture , biology , proteomics , tissue microarray , cell , antibody , chemistry , gene expression , gene , immunology , immunohistochemistry , genetics
Here we report the utility of Ab Microarray 500 to determine differences between normal and tumor cell lines. A human lymphoblastoid cell line (normal) and a human breast carcinoma cell line (grade 3, stage IIB tumor) from the same patient were compared. After protein extraction and labeling from the normal/tumor cell line pairs the protein extracts were used for the Ab Microarray 500 experiment and for the Western blot analysis. The results from the microarray were analyzed using an internally normalized ratio. The microarray results revealed twenty‐three proteins with elevated abundance in the tumor sample and nine proteins with elevated abundance in the normal sample. The data obtained from the array experiment was validated by western analyses with more than 80% correlation between the two methods.