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Structural correlation of glycoinositol phospholipids and pathological spectra of leishmaniasis from the old world parasite isolates
Author(s) -
Hwa Kuoyuan,
Khoo Kayhooi,
Chen ChanWei,
Chang KwangPoo
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.lb57
Subject(s) - parasite hosting , gene , biology , glycoconjugate , amino acid , galactose , pathological , peptide sequence , glycan , genetics , microbiology and biotechnology , biochemistry , glycoprotein , pathology , medicine , world wide web , computer science
Our objectives were to examine if there was any correlation between parasite surface glycoconjugates and pathological spectra in leishmaniasis and if beta‐galactofuranosyltransferase gene was differentially expressed in different pathological isolates. Thirty‐six field isolates were obtained from patients of cutaneous and visceral leishmaniasis. Glycoinositol phospholipids (GIPLs) were purified for structural analysis by mass spectrometry. Results suggested that GIPLs from most of the VL isolates had distinct structural spectra from the CL parasites. The correlation was statistically significant. And, no difference was found for N‐glycans. Composition analyses suggested that GIPLs from CL isolates had excess amount of galactose. To further investigate the molecular mechanism for the differential structures, the gene expression of beta‐galactofuranosyltransferase was examined by Northern analysis. The results indicated that no difference. However, our bioinformatics analysis on the amino acid sequence from three different pathological species indicated that there were several amino acid polymorphisms between the gruops. The differential structure might be due to variation of amino acid sequence of the gene (thus the enzymatic activities), not at the expression level. This work is supported by National Science Council (Taiwan)‐ NSC94‐2320‐B027‐001 to KY Hwa.