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The Anti‐Inflammatory Potential of Quercetin and L‐2‐Oxo‐thiazolidine‐4‐carboxylate (OTC) in Developing Scar Tissue
Author(s) -
Cox Nicole,
Abraham S,
Wiens R,
Rak M,
Gough KM,
Kulyk WM,
Juurlink BH
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.lb5-c
Subject(s) - quercetin , western blot , immunohistochemistry , saline , blot , histology , wound healing , chemistry , pathology , connective tissue , medicine , immunology , biochemistry , antioxidant , gene
The hypothesis is that quercetin and OTC have an inhibitory effect on the formation of peridural scar tissue following a spinal laminectomy, through an anti‐inflammatory mechanism. A spinal laminectomy was performed on male Wistar rats and the resultant scar tissues were studied at 3, 21, and 63 days post‐surgery. The groups of animals were treated with quercetin, OTC, or saline. Histological and immunocytochemical studies were performed to analyze the effects of quercetin and OTC in relation to the cellular inflammatory response. Synchrotron FTIR microspectroscopy was performed to determine the distribution of collagens and sugars in the scar tissue. Immunohistochemistry, northern dot blot and western blot were performed to determine the extent of collagen expression as well as to determine the levels of anti‐inflammatory cytokines such as TGF beta and to determine the levels of activated macrophages. Histology indicates that the OTC and quercetin treated samples are more organized morphologically and have lower cellularity in the healing wound that those treated with saline, suggestive of a greater cellular response and more fibroblast proliferation within the connective tissue. Northern dot blot analysis revealed that Collagen types I and III were expressed in scar tissues in each of the treatments. Immunohistochemistry was performed to determine various cytokines involved in anti‐inflammatory mechanism. Both quercetin and OTC have been shown to reduce the severity of scar tissue formation post‐injury, with OTC indicating a more positive response. This work is supported by NSERC CHRP.