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Oxygen tension modulates transpulmonary passage of 50μm solid microspheres under physiologic conditions in healthy rat lungs
Author(s) -
Eldridge Marlowe W,
Lovering Andrew T
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.lb31-c
Subject(s) - transpulmonary pressure , oxygen tension , microsphere , medicine , saline , perfusion , lung , anesthesia , albumin , room air distribution , oxygen , chemistry , lung volumes , physics , organic chemistry , chemical engineering , engineering , thermodynamics
We are accumulating direct evidence demonstrating the transpulmonary passage of large diameter microspheres in healthy isolated ventilated and perfused lungs of rats, dogs, baboons and humans. In this study we sought to determine the effect of oxygen tension on transpulmonary passage of 50 μm microspheres in healthy isolated ventilated and perfused rat lungs. Lungs were perfused at 20cm H 2 O with phosphate buffered saline, 10% albumin to prevent alveolar edema. Lungs were ventilated with either room air (21% O 2 ), 100% O 2 or 12% O 2 to a peak inspiratory pressure of 15cm H 2 O and 5cm H 2 O positive end expiratory pressure. Compared to room air (n=3), ~20 times more 50μm microspheres were able to traverse the lungs ventilated with 12% O 2 (n=2). In contrast, 50μm microspheres were not able to traverse the lungs ventilated with 100% O 2 (n=2). These data provide direct evidence that transpulmonary passage of large diameter microspheres is inversely proportional to oxygen tension under physiologic perfusion and inflation pressures in the healthy rat lung. Accordingly, the 100% O 2 technique would underestimate the contribution of arteriovenous anastomoses on gas exchange efficiency in normoxic conditions. Financial Support: NIH HL‐15469, T32 HL07654‐16, AHA 0550176Z.