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ERK MAP Kinase Pathway Activity After Heavy Resistance Exercise
Author(s) -
Taylor Lemuel W,
Willoughby Darryn S
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.lb28
Subject(s) - mapk/erk pathway , resistance training , squat , kinase , medicine , phosphorylation , analysis of variance , endocrinology , chemistry , cardiology , physical therapy , biochemistry
This study evaluated a single session of heavy resistance exercise on the activity of the ERK MAPK pathway. Seven males performed 3 sets of 8–10 repetitions at 75%–80% 1‐RM on the squat, leg press, and leg extension exercises (REX) while four served as a control (CON). Muscle biopsies were obtained 30 min pre‐exercise (30MPRE), 30 min post‐exercise (30MPST), and at 6 hr post‐exercise (6HPST). Total and phosporylated levels of MEK1 and ERK 1/2 were determined with ELISA and total and phosphorylated Raf‐1 determined with immunoblotting. Data were analyzed with two‐way repeated measures ANOVA (p < 0.05). No significant changes were noted for CON. However, when compared to pre‐exercise, REX underwent increases in Raf‐1, MEK 1, and ERK 1/2 activation at 30MPST (p < .05) that were also greater than CON. A single session of heavy resistance exercise increases the activity of the ERK MAPK pathway extending from Raf‐1 to ERK 1/2.