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Mechanism of synapse change in region around cerebral infarction area of rats
Author(s) -
tan laixun
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.lb23-d
Subject(s) - synaptophysin , synapse , cerebral infarction , glial fibrillary acidic protein , saline , astrocyte , infarction , medicine , endocrinology , chemistry , immunohistochemistry , pathology , biology , neuroscience , myocardial infarction , central nervous system , ischemia
AIM To investigate the vivid change of synapse and its molecular mechanisms in the region around the cerebral infarction area (area A) of the experimental rats. METHOD Respectively, after 7□14 and 28□of being made into experimental rat models of acute cerebral infarction, 120 rats were divided into 4 groups by chance: infarction□saline□inhibition and statin. The counts of astrocyte or synapse□the immunoreactivity of either glial fibrillary acidic protein (GFAP) or synaptophysin and the cholesterol content were observed by immunohistochemistry staining and high performance liquid chromatography (HPLC) in the area A respectively. RESULTS At the 7d□the 14d or the 28□after the infarction model was made, either the optical density of either GFAP or synaptophysin immunoreactivity and the cholesterol contents in area A showed significantly higher in either the infarction or the saline than in the inhibition ( p< 0.01), and the optical density of synaptophysin and the cholesterol contents were significantly lower in the mevastatin than in either the rehabilitation or the saline ( p< 0.01). CONCLUSION The synapse vivid may depend greatly on the astrocyte activity and especially on its secretion of cholesterol in the area A of the experimental rat with acute cerebral infarction.