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Mechanism for glutamate induced intracellular calcium change in cultured neurons of rats
Author(s) -
tan laixun
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.lb23-c
Subject(s) - ampa receptor , glutamate receptor , nmda receptor , calcium , intracellular , calcium in biology , extracellular , chemistry , long term depression , biophysics , silent synapse , receptor , glutamic acid , microbiology and biotechnology , neuroscience , biochemistry , biology , amino acid , organic chemistry
Objective To study the effect of glutamate on the intracellular calcium signal of cultured rat hippocampal neurons and its mechanism. Methods Using microfluorescent technique to detect the calcium signal and the effect of glutamate on it, observing its change upon the antagonization of NMDA and AMPA receptors simultaneously or respectively. Results Glutamate can increase the intracellular free Ca2+obviously, the antagonists of NMDA and AMPA receptors, the decrease of extracellular calcium concentration and the calcium chelator can attenuated the response at different extent Conclusion It is suggested that glutamate can affect intracellular calcium signal by complex mechanisms, the activation of NMDA and AMPA receptors is an important one of them.