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Yellow Curcumin Pigment is Effective Against Human Colon Cancer HCT 116 Cell Line
Author(s) -
Bassiouny Ahmad Raafat
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.lb20-b
Subject(s) - curcumin , pigment , colorectal cancer , cancer research , cancer , chemistry , medicine , biochemistry , organic chemistry
Colorectal cancer is the second leading cause of death in Egypt. We investigated the effect of curcumin on cell apoptosis in a cyclooxygenase‐2 (COX‐2) positive human colon cancer HCT 116 cell line. Curcumin induced apoptosis by inhibition of COX‐2 in both time‐ and concentration‐dependent manners. Curcumin treatment (0–60 μM) for 0–24 h led to early mitochondrial changes demonstrable as a decline of transmembranous potential (Delta) (psi) leading to cytosolic release of cytochrome‐C. We observed that p53 was highly expressed in HCT‐116 cells upon curcumin treatment while pro‐caspase‐3 and pro‐caspase‐9 were down regulated. Curcumin can inhibit the proliferation and induce apoptosis of HCT‐116 cells in vitro. This work was done at University of Texas Medical Branch.