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Measurement of hemoglobin saturation and hematocrit by a multispectral imaging approach
Author(s) -
StypRekowska Beata,
Reglin Bettina,
Disassa Nigussie M.,
Secomb Timothy W.,
Pries Axel R.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.lb17-b
Subject(s) - deoxygenated hemoglobin , hematocrit , hyperoxia , mesentery , intravital microscopy , microcirculation , hemoglobin , oxygen saturation , chemistry , oxygen , hypoxia (environmental) , pathology , materials science , medicine , biochemistry , organic chemistry
Determination of the local oxygen saturation in microvessels is relevant for intravital studies including angiogenesis, angioadaptation, tumor development or ischemia reperfusion injury. We adapted a multispectral approach, based on differences in absorption spectra between oxygenated and deoxygenated hemoglobin, for oxygen saturation (SO 2 ) and hematocrit measurement during trans‐ and epi‐ilumination intravital microscopy. Data can be obtained as 2D images of the observed area. For validation we performed in vivo experiments in rat mesentery under: hyperoxia (inspiratory O 2 concentration 100%, mesentery exposed to pure oxygen), normoxia (breathing room air, mesentery covered with oxygen‐impermeable film), hypoxia (inspiratory O 2 conc. 9% in N 2 , mesentery exposed to pure nitrogen), anoxia (superfusion of mesentery with Na‐dithionite). Using data obtained under hyperoxic and anoxic conditions as references, SO 2 values under normoxic control conditions averaged ~95% in arterioles and ~87% in venules. In conclusion, the presented method allows generation of intravascular SO 2 and hematocrit images for all vessels in a microscopic field of view during trans‐ and epi‐ilumination and in different tissues.