Premium
Specific expression of COX‐2 in type 1 muscle fibers
Author(s) -
Hyun Chang Lim,
Lee Hye Seung,
Park So Yeon,
Chung Jin Haeng,
Choe Gheeyoung
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.lb124-c
Subject(s) - immunohistochemistry , skeletal muscle , muscle biopsy , pathology , troponin , biopsy , medicine , troponin t , myopathy , muscle atrophy , atrophy , anatomy , myocardial infarction
A skeletal muscle biopsy is important for the diagnosis of muscle diseases. Frozen sections for histochemistry to determine patterns of muscle fiber types are essential for a proper pathological evaluation of skeletal muscle. Many Pathology Laboratories cannot perform histochemistry routinely by many limitations related to proper snap freezing. Recently, Troponin C and Troponin T have been developed for muscular fiber typing; however, they are not widely used. We incidentally discovered expression of cyclooxygenase ‐2 (COX‐2) in skeletal muscle tissue around breast cancer, and the “checkerboard” pattern of COX‐2 expression was suggestive of fiber typing of the skeletal muscle. We performed this study to verify whether COX‐2 is expressed in a specific muscle fiber type and to validate applicability of COX‐2 immunohistochemistry in the formalin‐fixed, paraffin‐embedded muscle biopsy specimens. We used 58 cases of muscle biopsy specimens consisting of 27 cases of neurogenic atrophy, 9 cases of muscular dystrophy, 8 cases of inflammatory myopathy, 7 cases of other muscular diseases, and 7 cases of nonspecific changes. Immunohistochemistry was performed with COX‐2, Troponin C and Troponin T monoclonal antibodies. Compared with immunohistochemical expression of Troponin‐C and Troponin‐T, and compared with histochemical studies of NADH‐TR and ATPase pH9.4, COX‐2 was specifically expressed in type 1 muscle fibers in various muscle diseases and normal skeletal muscle. Specific expression of COX‐2 in type 1 myofibers is a brand new finding hitherto undescribed in the literatures.