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Nutritional hypercholesterolemia increases hepatic inflammatory response to acute endotoxemia
Author(s) -
Desai Moreshwar S,
Mariscalco Mary Michelle,
Smith C Wayne
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.lb118-b
Subject(s) - lipopolysaccharide , medicine , liver injury , intraperitoneal injection , endocrinology , saline , inflammation , cholesterol , inflammatory response , analysis of variance , post hoc analysis
Dietary hypercholesterolemia and endotoxemia each cause liver injury. We hypothesize that nutritional hypercholesterolemia will potentiate liver injury from endotoxemia. Six week old male C57BL6 mice were randomized to purified high cholesterol (HC) or purified isocaloric chow diet. After 3wks intraperitoneal 10 mg/kg of lipopolysaccharide (LPS,E.coli O111B4) or saline was administered. After six hours mice were sacrificed and serum was collected for cholesterol and ALT. Liver was fixed and stained with H&E. The level of inflammation was assessed morphometrically as the volume percent of hepatic parenchyma comprised of inflammatory cells (% volume) per high power field. Statistics: ANOVA with Newman‐Keuls's post‐hoc testing. Results are mean± SEMConclusions Nutritional hypercholesterolemia potentiates endotoxin‐induced liver injury. Endotoxin potentiates the liver injury due to hypercholesterolemia. (supported by‐T32HL079‐01A2)