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Characteristics of in vitro prostate cancer models grown in human serum
Author(s) -
Hurdle Heather Louise,
Mazurak Vera C,
Clandinin M Thomas
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.lb103-a
Subject(s) - lncap , prostate cancer , angiogenesis , integrin , cell culture , vitronectin , flow cytometry , cell growth , biology , cancer research , cancer , andrology , immunology , medicine , receptor , genetics
Objective To compare growth, prostate serum antigen (PSA) production and integrin expression (α 2 β 1 , α v β 3 , vitronectin receptor VR) in established in vitro models of prostate cancer, when growth media is supplemented with human serum (HS) from healthy men instead of fetal calf serum (FCS), the current standard supplement. Methods The cell lines RWPE, 22Rv1, and LNCaP representing increasing degrees of invasiveness, were cultured in growth media containing 1% HS or 10% FCS. Growth, PSA levels and integrin expression were measured using trypan blue exclusion, ELISA assays, monoclonal antibodies and flow cytometry respectively. Results There were no differences in growth or PSA production by cell lines cultured in HS versus FCS. Expression of the α 2 β 1 integrin was observed to be significantly lower (p< 0.01) in 22Rv1s cultured in HS. 22Rv1 and LNCaP cell lines had lower α 2 β 1 expression compared to RWPE (p<0.05). Expression of the α v β 3 , VR was very minimal in all cell lines, although LNCaP expressed greater VR when cultured in HS compared to FCS (p = 0.03). Conclusions Cell lines representing human prostate cancer expressed differences in integrins reported to be involved in cell adhesion and angiogenesis when cultured using HS instead of FCS, despite the lack of change in growth and PSA production. This change in expression is significant in regard to standard culture methods and demonstrates that attempts to make culture methods more closely resemble human conditions must be considered. (Funding: CIHR grant; Elizabeth Donald Fellowship in Human Nutrition)