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Characterisation of DSS‐induced cytokine expression. Comparison of two indigestible‐carbohydrate‐enriched diets
Author(s) -
LERAY Véronique,
MOREAU Noëlle,
MARTIN Lucile,
CHAMP Martine,
DUMON Henri,
NGUYEN Patrick
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.lb100
Subject(s) - chemistry , endocrinology , medicine , messenger rna , interleukin 12 , cytokine , proinflammatory cytokine , inflammation , biochemistry , biology , in vitro , gene , cytotoxic t cell
Dextran sulfate sodium (DSS)‐induced colitis in rat is used as model for human IBD. The aim of the study was to assess cytokine expression in DSS‐induced colitis and to compare the protective effects of short‐chain fructo‐oligosaccharides (FOS), or a type 3 resistant starch (RS), which fermentability and fermentative profiles differ. Sprague‐Dawley rats received a solution of DSS in water in order to induce acute (A) inflammation (50 g/l for 7 d) then maintain it at chronic (C) level (30 g/l for 7 or 14 d; C7 and C14). The rats were fed either a basal diet (BD), or FOS diet, or RS diet. BD contained only 2 g cellulose/100 g DM as indigestible carbohydrates. FOS and RS diets provided 6 g/100 g DM of indigestible carbohydrates in addition to cellulose. mRNA expression of IFNα, IL1β, IL6, IL 8, IL12, IL13, TGFβ and TNFα was semi‐quantified by RT followed by real time‐PCR and compared with GAPdH expression, in control rats (no DSS), in BDA, BD‐C7 and BD‐C14 rats, and in rats fed either AR or FOS on day 21 (AR‐C14 and FOS‐C14). In BD‐A rats IFNα, IL1β, IL6, IL12, IL13 and TGFβ mRNA levels were higher than in control rats. In BD‐C7 rats, mRNA levels of all cytokines were still higher than controls. In BD‐C14 rats only IFNα, IL1β and IL13 mRNA levels differed from controls. In AR‐C14 rats, mRNA expression of IL1β, IL12, IL13 and TGFβ did not differ from controls, whereas they were higher in FOS‐C14 rats. These results suggest a role for RS but not FOS as modulator of inflammatory response. This could be related to their fermentation characteristics. RS would have beneficial effect in chronic colitis. This study was granted by National Veterinary School of Nantes.