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Effect of CLA on IL‐6 production of osteoblastic‐like cells treated with human prostate cancer conditioned media
Author(s) -
Durrer Katherine E.,
Shen ChwanLi
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a993-b
Subject(s) - conjugated linoleic acid , chemistry , downregulation and upregulation , cell growth , endocrinology , microbiology and biotechnology , medicine , andrology , biochemistry , linoleic acid , biology , fatty acid , gene
Our previous study has demonstrated that conjugated linoleic acid (t10,c12‐CLA) inhibited proliferation of human prostate carcinoma cells (PC‐3). Objective To evaluate the effect of conditioned medium collected from t10,c12‐CLA‐treated PC‐3 cells on the interleukin‐6 (IL‐6) production of mouse osteoblastic‐like cells (MC3T3‐E1). Methods PC‐3 cells were cultured in alpha‐MEM medium with 10% FBS. After confluence, PC‐3 cells were treated with and without CLA (40 microM) in a medium containing 0.25 % FBS for 48 hours, and such condition media (CM), PC‐3‐CM or PC‐3‐CLA‐CM, were collected. In another experiment, after reaching confluence, MC3T3‐E1 cells were treated with and without CLA (40 microM) in the PC‐3‐CM or PC‐3‐CLA‐CM for 24 hours. The supernatant was then collected for the determination of IL‐6. Results Both PC‐3‐CM and PC‐3‐CLA‐CM treatments significantly induced the IL‐6 production of MC3T3‐E1 compared to that in OB cells only. However, when MC3T3‐E1 cells treated with CLA for 24 hours in either PC‐3‐CM or PC‐3‐CLA‐CM condition significantly reduced IL‐6 production. This pilot study indicated that (i) although the cell proliferation of PC‐3 was inhibited by CLA, the secretory products in CM from PC‐3 still upregulated IL‐6 production of bone cells, and (ii) such upregulation of IL‐6 was diminished in bone cells treated with CLA for 24 hours.