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Characterization and expression of gene variants of hepcidin‐like antimicrobial peptides from black porgy (Acanthopagrus schlegelii B)
Author(s) -
Wang Kejian,
Yang Ming,
Chen Junhui,
Cai Jingjing,
Qu Haidong
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a973
Subject(s) - biology , hepcidin , spleen , complementary dna , microbiology and biotechnology , antimicrobial peptides , gastrointestinal tract , gene , perciformes , peptide , genetics , immunology , fishery , fish <actinopterygii> , biochemistry , inflammation
Hepcidin, an antimicrobial peptide, was firstly identified in fish in 2002 after human. It was speculated to be wildly spread among fish for surviving. In this study we present seven cDNA sequences (Genbank acc# AY669376 –82) amplified from liver, gill, spleen and skin of black porgy. These gene variants were tissue‐specifically distributed and divided into four groups: hepcidin1, hepcidin2 and hepcidin3 from the liver, hepcidin4 from the gill, hepcidin5 and hepcidin6 from skin, spleen and gill, and hepcidin7 from spleen and skin. Seven deduced prepropeptides (84–95 residues in length) have the same organization: signal peptide (24 aa), prodomain (40 aa) and mature peptide (20–31 aa), but their mature peptides were different from each other in constitutions and characteristics. Three genomic DNAs were determined (Genbank acc# DQ166812 –14) on hepcidin2, 3 and 4, all consisting of two introns and three exons. The expression levels of hepcidin2 and hepcidin7 were evaluated by Northern blot in multiple tissues of juvenile and one‐year mature fish. Hepcidin2 and hepcidin7 were all expressed at highest levels in the liver and kidney, and a higher level in the spleen of both juvenile and mature fish or in juvenile gastrointestinal tract but at different lower levels in heart, blood, brain, gill and skin. The results suggested that the seven hepcidins are expressed specifically in tissues at different developmental phases, and besides liver, kidney, spleen, gastrointestinal tract might be involved in the synthesis of the hepcidin. Supported by the grants (2003I005; 3502Z20021052).