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Caffeic acid suppresses UVB radiation‐induced expression of interleukin‐10 and activation of mitogen‐activated protein kinases in mouse
Author(s) -
Yang NingSun,
Staniforth Vanisree,
Chiu LuTang
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a940-a
Subject(s) - mapk/erk pathway , p38 mitogen activated protein kinases , kinase , immune system , chemistry , phosphorylation , protein kinase a , signal transduction , photoaging , transcription factor , microbiology and biotechnology , cancer research , biology , biochemistry , immunology , gene , genetics
Ultraviolet B (UVB) radiation present in sunlight causes sustained immune suppression, photocarcinogenesis and photoaging in humans. Interleukin‐10 (IL‐10) plays a critical role in UVB‐induced immune suppression by inhibiting cell‐mediated immune reactions. Mitogen‐activated protein kinases (MAPKs) have been implicated in UVB‐induced skin carcinogenesis. Caffeic acid (CA), a phenolic acid present in many dietary plants has been shown to confer antioxidant, anti‐inflammatory and anticancer activities. In the present study, we evaluated the protective effects of CA against UVB radiation‐induced IL‐10 expression and phosphorylation of MAPKs in mouse skin. An in vivo transgenic IL‐10 promoter‐luciferase reporter gene based assay revealed that CA inhibits the transcriptional activation of UVB‐induced IL‐10 promoter. This was further confirmed by significant inhibition of UVB radiation‐induced IL‐10 mRNA expression and protein production by CA. CA also significantly inhibited the UVB‐induced activation of MAPK signal transduction pathways, such as ERK, JNK and p38, and the downstream transcription factors AP‐1 and NF‐κB. The findings of our study suggest that CA may confer significant protection against UVB‐induced immune suppression and photocarcinogenesis in vivo and provide the possible underlying molecular basis for its actions. Supported by National Science and Technology Program for Agricultural Biotechnology (No. 94S‐0402).