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Differential effects of omega‐3 and omega‐6 fatty acids on global gene expression of breast cancer
Author(s) -
Chakraborty Nabarun,
Hammamieh Rasha,
Day Agnes,
Jett Marti
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a937-c
Subject(s) - carcinogenesis , breast cancer , metastasis , omega 3 fatty acid , cancer research , polyunsaturated fatty acid , cell growth , fatty acid , biology , cancer , bioinformatics , chemistry , biochemistry , docosahexaenoic acid , genetics
Experimental studies confirmed n‐6 type polyunsaturated fatty acid as pro‐carcinogenic factor and n‐3 fatty acid as cancer restraining agent; though their mode of action on tumor cells are still unclear. In the present project two members from each family of PUFAs, namely EPA and DHA from n‐3 PUFA family and, AA and LA from n‐6 PUFA family was selected to treat four breast cancer cell lines: MDA‐MB231, MDA‐MB435S, HCC2218 and MCF‐7 for two time period‐ 6hr and 24hr. The target was to review the contrasting effect of omega‐3 and omega‐6 fatty acids over carcinoma by studying genomic alteration in treated cancer cells. cDNA microarray was used as the primary platform. Subsequent data analysis showed the impact of fatty acid on tumor cells was more intense during shorter time course. Genes demonstrating largest divergence (0.05