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Reguration of gicerin gene expression by forskolin.
Author(s) -
Taira Eiichi,
Kohama Keiko
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a936-c
Subject(s) - forskolin , microbiology and biotechnology , messenger rna , northern blot , transmembrane protein , luciferase , biology , promoter , gene expression , gene , western blot , transfection , receptor , genetics
Gicerin is a cell adhesion that consists of five immunoglobulin‐like loop structures in an extracellular domain, a single transmembrane region, and a short cytoplasmic tail. Gicerin participates in nuerite extension and structural organization of nervous system. Expression of gicerin is highly observed during development and dramatically decreased after birth. Upstream region of the gene has a TATA less promoter with several Sp1 and a CRE binding site. Previously we found that the expression of gicerin in PC12 cells was regulated by NGF. Northern blot analysis revealed that NGF induced three folds increase of gicerin mRNA. To characterize its promoter region we examined the promoter activity in PC12 cells by luciferase assay. It showed that NGF‐induced transcriptional activity of the gicerin promoter was mediated by CRE. This time, we examined if the expression of gicerin was regulated by forskolin and found that exposure to forskolin significantly increase promoter activity of gicerin.