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Gene Expression Patterns and Pathway Functions of SEB Induced human PBMC
Author(s) -
Campbell Katherine C,
Maliborski Sarah,
Jett Marti,
Mendis Chanaka
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a934-a
Subject(s) - gene , signal transduction , gene expression , enterotoxin , biology , function (biology) , transduction (biophysics) , genetics , microbiology and biotechnology , escherichia coli , biochemistry
Staphylococcal Enterotoxin B (SEB) is one of several exotoxins produced by staphylococcus aureus that may be used as a bio‐weapon due its stability and fatality if inhaled. By investigating the SEB specific gene expression pattern we can locate and track the signal transduction events induced by SEB. Our research will not only track the multiple induced pathways such as 5‐LO, MAPK, and JNK, but will also confirm SEB specific gene expression patterns. We have observed the ability of specific inhibitors of key signal transduction pathway components such as p38 and 5‐LO inhibitors (SB203580 and MK591, respectively) block SEB induced signal activities with in minutes of exposure. We believe that these key components have the potential to function as diagnostic markers. We will assess the efficacy of the key components by investigating the alterations to the gene expression pattern of twenty‐five genes unique to SEB through reverse transcription polymerase chain reactions (RT‐PCR).Comparison of the complete gene profile of the two inhibitors will confirm the effectiveness of them to function as diagnostic markers.