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Early Intervention by Targeting Components that Inter‐connect Pathways in LPS Induced Human PBMCs
Author(s) -
Schiefelbein Emily Louise,
Turner Anne E,
Mendis Chanaka,
Jett Marti,
Mendis Chanaka
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a933
Subject(s) - signal transduction , gene , lipopolysaccharide , microbiology and biotechnology , computational biology , secretion , biology , upstream and downstream (dna) , upstream (networking) , immunology , genetics , biochemistry , computer science , computer network
Lipopolysaccharide (LPS) is an endotoxin that induces the secretion of pro‐inflammatory cytokines in many cells and is implicated in inducing septic shock in humans. Here we have followed a molecular approach by investigating the various signal transduction pathways induced by LPS. Previous investigations have revealed a set of LPS specific genes through micro‐array analysis. We further characterized the genes by using available pathway sites and literature searches have resulted in the identification of a number of pathways. Further investigation of each of the above pathways led us to find components that inter‐connect multiple pathways and believe that these targets as well as upstream and downstream components of the targets can be effectively utilized to block LPS induced cellular activities. We have seen a similar expression pattern and the time dependence of a set of genes previously identified through micro‐array analysis. We will assess the effectiveness of our targets by looking at the alterations of the set of LPS specific genes.