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Characterization and Expression of a Dehydrin Gene in Apple Tree (Malus domestica)
Author(s) -
GarciaBañuelos Monica Liliana,
VazquezMoreno Luz,
Gardea Alfonso A,
Winzerling Joy
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a923-a
Subject(s) - malus , biology , dormancy , complementary dna , gene , cdna library , gene expression , apple tree , microbiology and biotechnology , botany , genetics , germination
To gain a better understanding of genes associated with cold acclimation and dormancy in woody perennial apple trees ( Malus domestica ) from temperate zones, the gene expression of dehydrin, one of the important proteins involved in these processes was studied. A novel dehydrin gene , designated MdDhn, was isolated from Malus domestica (Golden Delicious) using RT‐PCR and RNA ligase mediated‐ rapid amplification of cDNA ends (RLM‐RACE). The full length of MdDhn cDNA consists of 993 nucleotides with an open reading frame of 687 b and a deduced amino acid sequence of 229 amino acid residues. The putative polypeptide is composed of two consensus Y‐segments, a stretch of serine residues and four Lysine‐rich repeats (Y 2 SK 4 ) a typical structure of dehydrins. The transcripts of MdDhn were highly accumulated in bark and bud tissues when the plant is dormant and cold hardy, but not during release of dormancy. Support for this research was from CONACyT 4074 4