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Calcium uptake and membrane potential in rat brain mitochondria during hypertension
Author(s) -
CalderónCortés Elizabeth,
CortésRojo Christian,
ClementeGuerrero Mónica,
VillalobosMolina Rafael,
SaavedraMolina Alfredo
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a922-b
Subject(s) - mitochondrion , calcium , membrane potential , endocrinology , homeostasis , medicine , inner mitochondrial membrane , intracellular , transmembrane protein , calcium in biology , calcium metabolism , chemistry , biology , biochemistry , receptor
Mitochondria contribute directly to the intracellular Ca 2+ homeostasis. Ca 2+ uptake is an energy‐requiring process driven by the electric potential established across the mitochondrial inner membrane. Several abnormalities in regard to Ca 2+ metabolism has been observed in spontaneously hypertensive rats (SHR). It is important to investigate if calcium handling and functionality in mitochondria might be altered in brain of hypertensive rats. Therefore, we investigated if membrane potential and Ca 2+ uptake change during hypertension and aging in SHR rats of 1 and 7 months‐age. We used the safranine technique to determine transmembrane potential and the rate of Ca 2+ transport was measured with the Ca 2+ indicator Arsenazo III. Transmembrane potential diminished more in SHR rats of 1 month‐age than 7 months old rats. Whereas, mitochondrial Ca 2+ uptake decreased in hypertensive brain rats of 1 month‐age compared brain mitochondria of SHR rats of 7 month old age. These data suggest that mitochondrial functionality is reduced with age and the establishment of hypertension. Acknowledgements The authors appreciate the partial economic support of grants from CONACYT (43705) and CIC‐UMSNH (2.16‐2005).