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Gene Expression Pattern and Pathway Functions of SEB and SEA Induced human PBMC
Author(s) -
Maliborski Sarah Lynn,
Mendis Chanaka,
Jett Marti
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.5.a912
Subject(s) - dna microarray , gene expression , microarray , gene , immune system , peripheral blood mononuclear cell , biology , toxic shock syndrome , microarray analysis techniques , computational biology , immunology , genetics , staphylococcus aureus , bacteria , in vitro
The Staphylococcal Enterotoxins are a family of related toxins whose biological toxicities include staphylococcal food poisoning, various skin disorders, toxic shock syndrome, and possible involvement in auto‐immune disorders. We focused on exploring the differences in physical symptoms by studying, identifying, and comparing, the gene expression profile of SEB and SEA, and the most potent time point/concentration of SEA exposure. We utilized customized microarrays to study the differences in the gene expression pattern of SEB and SEA as well as to figure out the optimum SEA exposure (time and concentration). Human peripheral mononuclear cells were either exposed to SEA or SEB at various concentrations at multiple time points (2–20hours) and RNA samples were extracted and analyzed through an agilent protein/ DNA analyzer. The relative florescent intensities between the control and experimental samples for each gene represented on the microarray was determined through genespring software. The experiments allowed us not only to identify the optimum concentration and the time dependence of SEA exposure but also to differentiate SEA and SEB exposure by looking at the gene profiles. We believe that our research will provide crucial information needed to differentiate and better understand the mechanisms induced by the two toxins.